4D embryogenesis study using X-Ray micro-CT

May 23, 2019

Czech Technical University in Prague : Conference proceedings, 2019.


In recent years, X-ray micro-tomography has found its application in many previously unthinkable scientific fields. Thanks to the development of nano-focus sources, 3D data with a spatial resolution of less than 1 um can be obtained in radiographic laboratories and new digital detectors allow the imaging of structures made of materials with very similar X-ray absorption. In this work, we focus on the possibilities of displaying soft tissues of mouse embryos, where it is possible to monitor embryonic development by measuring various developmental stages. Various ways of stabilization, contrast agents, and mechanical fixation methods have been tried for selected stages E14, E18 and P1, which consequently led to the establishment of an appropriate protocol for measuring this type of samples. The results of tomographic measurements of samples stabilized in ethanol series, contrasted with Lugol's solution and mechanically fixed by agarose gel, enable identification of individual organs and follow their growth in the monitored time period.

How Our Software Was Used

Dragonfly was used to visualize 3D sample volumes and individual planes of a laboratory mouse's CT-data.

Author Affiliation

(1) Czech Technical University in Prague, Faculty of Nuclear Sciences and Physical Engineering, Prague, Czech Republic
(2) Czech Technical University in Prague, Institute of Experimental and Applied Physics, Prague, Czech Republic